
SK-MES-1
ACC 353
Cell line:
SK-MES-1
DSMZ no.:
ACC 353
Species:
human (Homo sapiens)
Cell type:
lung squamous cell carcinoma
Origin:
established from the pleural effusion of a 65-year-old man with lung squamous cell carcinoma in 1970; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelial-like adherent cells growing as monolayers; image
Medium:
80-90% DMEM + 10-20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture about 1:3 to 1:5 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 44% polyploidy - 54-60<2n>X, -Y, +1, +1, -2, +4, +5, +7, +7, +11, +13, +19, +22, +5mar, add(X)(p21), i(1q), del(1)(q12)x1-2, add(2)(q37), del(3)(p22p26), add(4)(q32), der(5)t(5;6)(p15;p11), del(5)(q13), add(7)(q11;abr), add(11)(q2)x2, add(13)(p12)x1-2, add(13)(p12), add(15)(p12), der(16)t(8;16)(q12;q23), del(17)(p11), add(19)(p13)x2-3 - sideline with additional der(7;8)(q10;p10), add(22)(q13), der(11)dup(11)(q13q22) - carries 3p-, 5q-, +7, 17p- associated with NSCLC - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list