HCT-15
ACC 357
Cell line:
HCT-15
DSMZ no.:
ACC 357
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from a colorectal adenocarcinoma; HCT-15 is a sister cell line of the cell line DLD-1 (DSMZ ACC 278)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out 2 x 106 cells in a 80 cm2 flask; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA; maximal density at ca. 50 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-25 hours
Harvest:
harvest of ca. 25 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line DLD-1 (ACC 278)
Cytogenetics:
human pseudodiploid karyotype with 16% polyploidy - 46<2n>XYq+, t(8;17) (p23;q12/21), inv(11)(p14.2;q13) - stable karyotypic differences cf DLD-1 - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list