Leibniz Institute DSMZ: Details

Cell line:

CGTH-W-1 - Discontinued

DSMZ no.:

ACC 360

Species:

human (Homo sapiens)

Cell type:

thyroid carcinoma (cell line SW-579)

Origin:

Caution: As determined by STR analysis, CGTH-W-1 is genetically identical to the human thyroid carcinoma cell line SW-579.

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

adherent large epitheloid cells with long processes growing in monolayers with multilayer foci; image ; image ; image ; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

split confluent culture 1:4 to 1:10 every 2-5 days using trypsin/EDTA; seed out at ca. 1-4 x 10⁶ cells/175 cm²

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 20-25 hours

Harvest:

cell harvest of ca. 30-50 x 10⁶ cells/175 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in the STR profile of cell line SW-579.

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

see price list

Print data sheet