Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1971; sister cell line of MOLT-3 (DSMZ ACC 84). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
80% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.3 x 106 cells/ml; split ratio of ca. 1:2 to 1:3 every 2-3 days; viability may transiently decrease after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
maximal density at ca. 1.3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2(+), CD3(+), CD4(+), CD5+, CD6+, CD7+, CD8(+), CD13-, CD19-, CD34(+), TCRalpha/beta-,
TCRgamma/delta-, cyCD3+;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertetraploid karyotype - 89-99<4n>XXYY, +4, +7, +8, +20, +20, del(6)(q16)x2, der(7)t(7;7)(p15;q11)x2 - closely related to MOLT-3
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
Growing culture
(please inquire for exact delivery time) | | 1040,- € |
| Frozen culture | | 520,- € |
| DNA isolated from cell line (25 µg) | | 650,- € |
| DNA isolated from cell line (5 µg) | | 160,- € |
see price list
