KYSE-70
ACC 363
Cell line:
KYSE-70
DSMZ no.:
ACC 363
Species:
human (Homo sapiens)
Cell type:
esophageal squamous cell carcinoma
Origin:
established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 77-year-old man prior to treatment (depth of invasion was not beyond the muscularis propria); described in the literature to carry mutations in the TP53 gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 4-6 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask); prior to using trypsin, cells should be washed twice with PBS)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
cell harvest of ca. 15-20 x 106 cells/80cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 8% polyploidy - 60(54-63)<3n>XXX, -Y, -2, -3, -3, -4, -6, +7, -8, -9, -10, -13, -14, -15, -16, -16, -16, -17, -18, -18, -21, -22, +10mar, del(X)(q25), add(1)(p11), add(2)(q33), del(2)(q33), del(3)(q13), add(4)(q35), add(5)(p15), del(5)(q14), add(7)(q36), del(7)(p11), del(8)(p22.2), qdp(9)(p11p24), add(10)(q25), add(11)(p?13), add(19)(p13), i(?22p)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV +, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list