MEG-01
ACC 364
Cell line:
MEG-01
DSMZ no.:
ACC 364
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in megakaryocytic blast crisis
Origin:
established from the bone marrow of a 55-year-old man with chronic myeloid leukemia (CML) in megakaryocytic blast crisis in 1983; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3 - 0.5 x 106 cells/ml; split saturated culture 1:3 to 1:4 every 3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
maximal density at ca. 1.3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD15 +, CD19 -, CD33 +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 12% polyploidy - 54(53-56)<2n>XY, +6, +19, +19, +21, +3-4mar, t(1;15)(p13;p13), ?inv(3)(p25q26), i(4q), add(5)(p15), der(9)t(9;22)(q34;q11)x2, add(10)(p14), dup(13)(q13q33-34), add(14)(p11), der(22)t(9;22)(q34;q11) - r/dmin x1 present at 57% - sideline with i(5)(q10)del(5)(q11q13) instead of add(5) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list