
BFTC-909
ACC 367
Cell line:
BFTC-909
DSMZ no.:
ACC 367
Species:
human (Homo sapiens)
Cell type:
kidney transitional cell carcinoma
Origin:
established from the sarcomatoid component of a grade III transitional cell carcinoma of the renal pelvis of a 64-year-old man in 1990; patient lived in a blackfoot disease endemic area in Taiwan probably related to TCC
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelial-like (spindle) cells growing in monolayers; cells contain considerable amounts of small, dark granules; image
Medium:
80-90% DMEM + 10-20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:3 to 1:5 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 3-5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriplod karyotype with 32% polyploidy - 66-79<3n>XX, -Y, +1, +3, +5, +7, -9, -11, +12, +17, -18, +19, +20, +21, +mar, add(1)(p34)x2, del(2)(p16), +dic(2;9)(q25;p22)x2, add(3)(p24)x2, add(6)(p23)x2, i(7p)x2, i(8q)x2, del(11)(q?22q23), add(12)(q24), add(19)(q13)x2 - 2-4 dmin present in most cells - gain of ch 7 and deletions of 9p typical in TCC - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list