KMOE-2
ACC 37
Cell line:
KMOE-2
DSMZ no.:
ACC 37
Species:
human (Homo sapiens)
Cell type:
acute erythremia
Origin:
established from the peripheral blood of a 2-year-old girl with acute erythremia (Di Gugliemo's disease, erythroblastosis) at diagnosis in 1978; cells were described in the literature to produce hemoglobin
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.3-0.4 x 106 cells/ml; maintain at 0.2-0.3 x 106 cells/ml; note that after thawing cell viability may be as low as 50%, but cells will recover quickly within a few days
Incubation:
at 37 °C with 5% CO2
Doubling time:
doubling of ca. 40-50 hours
Harvest:
maximal density of 0.6 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD15 -, CD19 -, CD33 +, CD34 -, CD71 +, CD235a -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype (mode weak) with 6% polyploidy - 82(80-88)<4n>XX, -X, -X, -10, -13, -14, -16, -18, -20, -22, +3mar, der(1)t(1;?)(q21;?)x2, del(7)(p15)x2, del(9)(p12), i(17q) - karyotype appears to represent derivative of that originally reported
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list