TCC-SUP
ACC 377
Cell line:
TCC-SUP
DSMZ no.:
ACC 377
Species:
human (Homo sapiens)
Cell type:
urinary bladder transitional cell carcinoma
Origin:
established from the tumor specimen resected from the urinary bladder transitional cell carcinoma (undifferentiated, grade IV) of a 67-year-old woman in 1974 (with therapy)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-85% DMEM (4.5 g/L glucose) + 15-20% h.i. FBS
Subculture:
split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 6 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 8% polyploidy - 67(58-67)<3n>XX, -X, +1, -2, -4, +5, -9, -12, -18, -21, +1-2mar, der(1;21)(q10;q10), del(1)(q13), i(5p)x3, del(6)(q14), add(6)(p23)/der(6)t(3;6)(p22;p22), add(6)(p22/23), del(10)(p11), add(20)(q12), add(21)(p11/12) - i(5p) and rearrangements of chs 1 and 3p recurrent in bladder TCC - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list