
PF-382
ACC 38
Cell line:
PF-382
DSMZ no.:
ACC 38
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the pleural effusion of a 6-year-old girl with acute lymphoblastic leukemia (ALL) in second relapse
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
maximal density of about 3-4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 9% near-tetraploid and 15% polyploid sidelines - 45/46(43-47)<2n>X/XX, -10, +14 - sideline with +add(?15)(p11), add(1)(p32) - identity confirmed
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list