KYSE-410
ACC 381
Cell line:
KYSE-410
DSMZ no.:
ACC 381
Species:
human (Homo sapiens)
Cell type:
esophageal squamous cell carcinoma
Origin:
established from the poorly differentiated invasive squamous cell carcinoma resected from the cervical esophagus of a 51-year-old man prior to treatment (tumor invasion into the adventitia was obvious); described in the literature to carry TP53 and KRAS mutations and to overexpress hast-1 (= heparin-binding growth factor) and cyclin D1
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 45 hours
Harvest:
cell harvest of ca. 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype with 4% polyploidy - 79-91<4n>Xder(X), -10, -18, -19, -21, -22, -22, +6mar, add(X)(qter), add(1)(p11)/del(1)(p13), add(3)(q21)x1-2, add(5)(p13), der(6)t(4;6)(q12;q14)x1-2, dup(7)(p12p14), der(7)del(7)(p12p21)t(7;9)(q33;q10), add(8)(p12)x1-2, add(9)(q34), add(10)(p14), add(11)(p11), add(12)(q24)x1-2, add(12)(p11-12)x2, del(12)(q14), i(13q)x2, i(14q)x1-2, add(14)(p11), add(19)(p13), add(?20)(q13), add(21)(p11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list