EGI-1
ACC 385
Cell line:
EGI-1
DSMZ no.:
ACC 385
Species:
human (Homo sapiens)
Cell type:
bile duct carcinoma
Origin:
established from the solid tumor of a 52-year-old man with advanced stage malignant bile duct carcinoma (patient had not received any chemotherapy prior to cell explantation) in 1984 and three consecutive passages in female nu/nu mice (NMRI); primary tumor histology: large cell adenocarcinoma of low differentiation, metastasizing (ascites in patient); human character of cell line confirmed by karyotype in passage #34 of in vitro culture (1986)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% MEM + 10% h.i. FBS + 2x MEM amino acids (both essential and non-essential) + 4 mM L-glutamine + 1 mM sodium pyruvate
Subculture:
seed out at ca. 1-2 x 106 cells/175 cm2;
split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA (wash twice with PBS, more than 20 min trypsin/EDTA treatment might be required on confluent cultures)
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 45-50 hours
Harvest:
cell harvest of ca. 10-12 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid with 8% polyploidy - 70(68-72)<3n>XYY, -8, -10, +11, -12, -15, +21, +5mar, del(4)(q22), i(5q), i(6p)x2, add(18)(q23)x2, del(21)(q22) - rearrangements of 18q and 21q implicated in gall bladder carcinoma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list