KYSE-450
ACC 387
Cell line:
KYSE-450
DSMZ no.:
ACC 387
Species:
human (Homo sapiens)
Cell type:
esophageal squamous cell carcinoma
Origin:
established from the well differentiated invasive squamous cell carcinoma resected from middle intra-thoracic esophagus of a 59-year-old man prior to treatment (depth of invasion was not beyond the submucosa); described as carrying p53 mutation and amplification of the oncogene MYC
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
45% RPMI 1640 + 45% Ham's F12 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/25 cm2; split confluent culture 1:8 to 1:10 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 5 x 106 cells/25 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), thereafter negative in DAPI, microbiological culture, RNA hybridization, and repeatedly in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 6.7% polyploidy - 57(49-57)<2n>, -Y, +1, +3, +3, +7, +12, +17, -18, +7mar, der(1)t(1;6)(p22;q11), add(1)(p32/34), del(2)(p23), del(3)(p22), del(4)(q21), add(5)(q35), del(6)(q25), add(7)(p22), add(8)(p23), add(9)(q34), del(11)(q14q21), add(12)(q14), del(12)(p11p12), der(14)t(8;14)(q21;p11), del(17)(p11), der(19)t(1;19)(q11;q12) - sideline with add(11)(q?23) - extensive subclonal rearrangements of ch 7
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list