MSTO-211H
ACC 390
Cell line:
MSTO-211H
DSMZ no.:
ACC 390
Species:
human (Homo sapiens)
Cell type:
mesothelioma
Origin:
established from the pleural effusion of a 62-year-old man with mesothelioma (malignant biphasic) who did not have any prior therapy
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:5 every 3 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 in 10 ml medium
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype - 79(77-80)<3n>XYY/XXYY, +2, +4, +7, +7, +7, -10, +12, +13, +14, -15, -15, +16, +16, +16, -17, +18, -22, +1-2mar, 1-20dmin - add(1)(p35-36), add(1)(p34), der(4)t(4;17)(p14;q21)x2, del(6)(q25)x2, del(7)(q11), del(7)(q21.2)x2, inv(9)(p24q34), del(10)(q24), add(14)(p11), add(15)(p11), i?dic(16)(q10)x2 - sideline with der(6;9)(p10;q10) - high 7p copy number associated with poor prognosis mesothelioma - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list