U-698-M
ACC 4
Cell line:
U-698-M
DSMZ no.:
ACC 4
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the involved tonsil of a 7-year-old boy with lymphoblastic lymphoma at diagnosis prior to therapy in 1972
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.7 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; some cells adhere to plastic, but can be detached mechanically (trypsin not necessary)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximal density at 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Immunology:
CD3-, CD10+, CD13-, CD19+, CD20+, CD34-,
CD37+, CD38+, CD80-, CD138(+), HLA-DR+,
sIgG-, sIgM+, sI-gkappa+, sIg-lambda-, cIgG-, cIgM+, cIg-kappa+, cIg-lambda-, cyCD79a+; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 5.5% polyploidy - 49(44-50)<2n>XY, +3, +7, -14, +mar, dup(1)(q43q21.2), der(2)t(2;3)(p16;p11), add(3)(p11), del(6)(q15q22), del(9)(p22), dup(11)(q23q13), add(13)(p12), add(16)(q24) - carries large submetacentric dup(1) marker
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list