CTV-1
ACC 40
Cell line:
CTV-1
DSMZ no.:
ACC 40
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
allegedly established from the peripheral blood of a 40-year-old man with acute monoblastic leukemia (AML M5) at relapse in 1982 (according to original publication allegedly derived from a woman); however, recent new data suggest derivation from T-ALL (acute lymphoblastic leukemia): (1) T-ALL-associated gene expression signature (Andersson et al., Leukemia 19: 1042-1050, 2005); (2) own FISH analysis confirming t(1;7) effecting TAL1-TRB fusion; (3) own reanalysis of the immunoprofile
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.8-1.0 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml;
split 1:2 to 1:4 every 3-4 days; after thawing, culture should first be started with 20% FBS and best in 24-well-plates
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
maximal density at about 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 (+), CD7 +, CD8 -, CD13 -, CD15 +, CD19 -, CD33 -, CD34 -, HLA-DR -, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near tetraploid karyotype with 9% polyploidy - 92(89-94)<4n>XX/XXY/XXYY, -1, +6, +6, -17, t(1;7)(p34.2;q34)x2, del(3)(p21), i(6q)x2, t(12;16)(q24.32;q11)x2
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list