Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG, EBV-negative) in leukemic transformation (refractory, terminal) in 1986. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
80-90% Iscove's MDM + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.7 x 106 cells/well; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 every 2-3 days; viability may be low initially after thawing; it is recommended to cultivate the cells in 24-well-plates during the first week and to exchange medium with dilution; cells may be partially (lightly) adherent but can be flushed off by pipetting
Incubation:
at 37 °C with 5% CO2
Doubling time:
35-80 hours (depending on cell density)
Harvest:
maximal density at about 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded aneuploid hypertriploid karyotype - 79(72-79)<3n>XX, -X,-1, +3, +4, +5, +6, +8, +11, +12, -13, +14, +15, +16, +18, -19, +20, +21, +22, +dminx1-4, add(1)(p11-21), del(3)(q22), der(4)add(4)(p1?)t(4;11)(11;?)(q33;q13)(q24;?)x2, der(5)t(1;5)(p11;q35), del(6)(q15)x1-2, del(8)(p21)x1-2, add(9)(q34-35)x1-2, t(12;13)(p12;q11)x2 - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
