MFE-280
ACC 410
Cell line:
MFE-280
DSMZ no.:
ACC 410
Species:
human (Homo sapiens)
Cell type:
endometrium adenocarcinoma
Origin:
established from tumor tissue from a 77-year-old woman with recurrence of endometrial carcinoma (adenomatous, partly papillary, grade G3) in 1990; described as forming heterotransplantable tumors in nude mice and as carrying receptors for progesterone
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
45% RPMI 1640 + 45% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite
Subculture:
seed out at 2-6 x 106 cells/75 cm2; about 4 hours after thawing, floating cells and medium should be decanted into a second flask; split confluent culture 1:2 to 1:6 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-90 hours
Harvest:
cell harvest of ca. 20 x 106 cells/75 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 24% polyploidy - 52(48-52)<2n>X, -X, +1, +1, -2, +9, -10, -12, -13, -16, -17, -17, -18, -19, -20, -22, +18mar, add(1)(p34), add(1)(p3?), add(1)(q11), add(3)(q13), add(3)(q27-29), add(4)(p14), der(9)t(9;13)(p11;q11), add(15)(p11) - near diploidy and gain of 1q typical in endometrial carcinoma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list