647-V
ACC 414
Cell line:
647-V
DSMZ no.:
ACC 414
Species:
human (Homo sapiens)
Cell type:
urothelial bladder carcinoma
Origin:
established from a male patient with a primary urothelial bladder carcinoma (malignant, grade 2)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% DMEM + 15% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:4 to 1:8 every 3-4 days using trypsin/EDTA (wash twice with PBS before trypsination)
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32), del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24), del(11)(q22q24), del(13)(q?11q?14), der(19)t(12;19)(q13;q13), add(21)(p11) - i(5p) associated with bladder cancer
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list