RT-112
ACC 418
Cell line:
RT-112
DSMZ no.:
ACC 418
Species:
human (Homo sapiens)
Cell type:
urinary bladder transitional cell carcinoma
Origin:
established from the transitional cell carcinoma (histological grade G2) excised from a woman (age unknown) with untreated primary urinary bladder carcinoma in 1973; described in the literature to form tumors in nude mice
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split culture prior to confluence 1:2 to 1:4 every 2-5 days using extended trypsin/EDTA treatment (5-10 min)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
cell harvest of ca. 20-30 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 47(44-47)<2n>XX/XXX, +X, +10, +11, +14, -17, +mar, add(2)(q3?2), del(3)(p14p24.1), i(4p), add(6)(q2?), i(8q), del(9)(p21p22), add(10)(p12), der(11)t(3;11)(q11-13;p15), add(17)(p1?1), add(18)(q11-22) - sideline with: idem, del(9)(p11), del(11)(q23), add(12)(q24.32), del(13)(q11q14), add(21)(p11) - resembles published karyotypes
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list