1G1
ACC 424
Cell line:
1G1
DSMZ no.:
ACC 424
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-chicken dorsal retina cytoplasmic membranes, E12)
Origin:
cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with enriched retinal cell membranes; described as secreting a mouse monoclonal antibody (IgM) reacting with the chicken dorsal retina cytoplasmic membranes (E12) and binding outer photoreceptor elements of adult vertebrate retina (inclusive chick, mouse, rat)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
spheroidal cells growing singly in suspension, or occasionally loosely adherent
Medium:
90% RPMI 1640 + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing)
Subculture:
maintain at ca. 0.1-0.3 x 106 cells/ml; split saturated culture 1:3 to 1:10 every 2-5 days (some cells attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca. 0.3 x 106 cells/ml; a maximal density of 1.0 x 106 cells/ml should not be exceeded
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgM monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypertetraploid karyotype - 94-102<4n> - no centric fusion markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list