SK-MM-2
ACC 430
Cell line:
SK-MM-2
DSMZ no.:
ACC 430
Species:
human (Homo sapiens)
Cell type:
plasma cell leukemia
Origin:
established from the peripheral blood of a 54-year-old man with plasma cell leukemia (Igkappa) (refractory, terminal state) in 1982; plasma cell leukemia is related to multiple myeloma
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells are difficult to culture; seed out at ca. 1.0 x 106 cells/ml; cells grow slowly during the first week after recovery from liquid nitrogen; do not culture below 0.6 x 106 cells/ml; split saturated culture 1:2 every 4-5 days; long-term cultivation might lead to accumulation of microvesicles without affecting viability and proliferation
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 100 hours
Harvest:
cell harvest of ca. 1.3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization and PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR -, sm/cyIgG -, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human bimodal hypodiploid/hypotetraploid karyotype with 9% polyploidy (including a subpopulation with 32-64N) - 42(38-43)<2n>XX, -13, -18, -22, del(1)(p32p36), der(2)t(1;2)(p32;q3?1)t(1;13)(p36q1?4), der(4;14)(4pter->4p15::4p11->4p10::14q10->14q22::4q11-12->4qter), del(6)(q15), i(8q), der(14)t(11;14)(q13;q32) (46%) - 84(78-84)<4n>idemx2 (45%) - carries non-reciprocal t(11;14) causing rearrangement of CCND1 (BCL1) associated with chronic lymphoproliferative disorders - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list