SK-MM-2

ACC 430

Cell line:

SK-MM-2

DSMZ no.:

ACC 430

Species:

human (Homo sapiens)

Cell type:

plasma cell leukemia

Origin:

established from the peripheral blood of a 54-year-old man with plasma cell leukemia (Igkappa) (refractory, terminal state) in 1982; plasma cell leukemia is related to multiple myeloma

Reference(s):

14486, 14521, 14809

Biosafety level:

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

round single cells growing in suspension, cells may contain microvesicles; image; image; image; image

Medium:

80% RPMI 1640 + 20% h.i. FBS

Subculture:

cells are difficult to culture; seed out at ca. 1.0 x 10⁶ cells/ml; cells grow slowly during the first week after recovery from liquid nitrogen; do not culture below 0.6 x 10⁶ cells/ml; split saturated culture 1:2 every 4-5 days; long-term cultivation might lead to accumulation of microvesicles without affecting viability and proliferation

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 100 hours

Harvest:

cell harvest of ca. 1.3 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization and PCR assays

Immunology:

CD3-, CD10-, CD13-, CD19-, CD20-, CD34-, CD37-, CD38(+), CD80-, CD138+, HLA-DR-, cyCD79a-, cIgG-, cIgM-, cIg-kappa+, cIg-lambda-, sIgG-, sIgM-, sIg-kappa-, sIg-lambda-; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human bimodal hypodiploid/hypotetraploid karyotype with 9% polyploidy (including a subpopulation with 32-64N) - 42(38-43)<2n>XX, -13, -18, -22, del(1)(p32p36), der(2)t(1;2)(p32;q3?1)t(1;13)(p36q1?4), der(4;14)(4pter->4p15::4p11->4p10::14q10->14q22::4q11-12->4qter), del(6)(q15), i(8q), der(14)t(11;14)(q13;q32) (46%) - 84(78-84)<4n>idemx2 (45%) - carries non-reciprocal t(11;14) causing rearrangement of CCND1 (BCL1) associated with chronic lymphoproliferative disorders - resembles published karyotype

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Growing culture (please inquire for exact delivery time)		1040,- €
Frozen culture		520,- €
DNA isolated from cell line (25 µg)		650,- €
DNA isolated from cell line (5 µg)		160,- €

see price list

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