8-MG-BA
ACC 432
Cell line:
8-MG-BA
DSMZ no.:
ACC 432
Species:
human (Homo sapiens)
Cell type:
glioblastoma
Origin:
established from the frontal brain lobe of a 54-year-old woman with glioblastoma multiforme in 1989 (pre-treatment)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-85% MEM (with Earle's salts) + 15-20% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2; optimal density at ca. 5-10 x 106/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 100 hours
Harvest:
cell harvest of maximal 15 x 106/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 15% polyploidy - 52(47-52)<2n>X, -X, +1, +1, +3, +5, +6, +7, +8, -19, +2-4mar, add(1)(p34-36;hsr), add(1)(p34-36), der(1)t(1;17)(q11-12;q12-21), der(3;5)(q10;q10), i(5p), del(6)(q24-25), del(7)(p14), i(7p), i(8q), add(8)(p21)x1-2, del(9)(q32q34)/add(9)(q32), add(11)(?q13), del(12)(q14), del(18)(q22.2), add(19)(p13.1) - sideline with del(3)(p11), der(6;9)(p10;q10) - 2-4 acf present in 31% cells
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list