HSB-2

human (Homo sapiens)

T cell leukemia

established in 1966 from the peripheral blood of an 11-year-old boy with lymphosarcoma that had progressed to acute lymphoblastic leukemia (ALL); cell line was established and initially passaged in newborn hamsters; cells were described as carrying the t(1;7)(p34;q34) involving the LCK and TRB (LCK-TCRB) genes and a submicroscopic del(1)(p32) leading to the STIL-TAL1 (SIL-SCL) fusion gene; one of the oldest immature T cell lines, also known in the literature as CCRF-HSB-2

14345, 14346

1

A

round to polygonal cells, growing singly or in dense clumps in suspension; image

90% RPMI 1640 + 10% h.i. FBS

seed out at ca. 0.3-0.4 x 10⁶ cells/ml; maintain at ca. 0.4-1.0 x 10⁶ cells/ml; split saturated culture 1:3 every 2-3 days; maximal density at ca. 1.3 x 10⁶ cells/ml

at 37 °C with 5% CO₂

ca. 25 hours

cell harvest of ca. 0.8 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays

CD2-, CD3-, CD4-, CD5+, CD6+, CD7+, CD8+, CD13-, CD15-, CD19-, CD33-, CD34-, TCRalpha/beta-, TCRgamma/delta-, cyCD3(+); image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human pseudodiploid karyotype with 4% polyploidy - 46(42-46)<2n>XY, t(1;7)(p34;q35), carries t(1;7) effecting LCK-TRB (LCK-TCRB) fusion, resembles published karyotype

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Growing culture   (please inquire for exact delivery time)		1040,- €
Frozen culture		520,- €
DNA isolated from cell line (25 µg)		650,- €
DNA isolated from cell line (5 µg)		160,- €

see price list

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