HSB-2
ACC 435
Cell line:
HSB-2
DSMZ no.:
ACC 435
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established in 1966 from the peripheral blood of an 11-year-old boy with lymphosarcoma that had progressed to acute lymphoblastic leukemia (ALL); cell line was established and initially passaged in newborn hamsters; cells were described as carrying the t(1;7)(p34;q34) involving the LCK and TRB (LCK-TCRB) genes and a submicroscopic del(1)(p32) leading to the STIL-TAL1 (SIL-SCL) fusion gene; one of the oldest immature T cell lines, also known in the literature as CCRF-HSB-2
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round to polygonal cells, growing singly or in dense clumps in suspension; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3-0.4 x 106 cells/ml; maintain at ca. 0.4-1.0 x 106 cells/ml; split saturated culture 1:3 every 2-3 days; maximal density at ca. 1.3 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 hours
Harvest:
cell harvest of ca. 0.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype with 4% polyploidy - 46(42-46)<2n>XY, t(1;7)(p34;q35), carries t(1;7) effecting LCK-TRB (LCK-TCRB) fusion, resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list