CAL-12T
ACC 443
Cell line:
CAL-12T
DSMZ no.:
ACC 443
Species:
human (Homo sapiens)
Cell type:
non-small cell lung carcinoma
Origin:
established from a male patient with a non-small cell lung carcinoma in 1981; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2 ; split confluent culture 1:2 to 1:3 every second day using trypsin/EDTA (incubation with trypsin/EDTA for about 15-20 min)
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 12% polyploidy - 59(57-59)<3n>X, +1, -2, -4, -5, -6, -6, -7, -11, -12, -13, -16, -16, -18, +20, +4-5mar, der(1;18)(p10;q10), der(1)t(X;1)(?;p?21)t(1;?)(q1?1;?), der(1)(q12->q10::p10->pter), add(3)(p11), add(4)(p11), add(5)(p15), add(9)(q1?)x1-2, add(11)(q24), dup(11)(q13q24), del(12)(p12), i(14q), add(19)(q11), i(21q)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list