CAL-33
ACC 447
Cell line:
CAL-33
DSMZ no.:
ACC 447
Species:
human (Homo sapiens)
Cell type:
tongue squamous cell carcinoma
Origin:
established from the surgically removed fragment of a tongue lesion from a 69-year-old man with moderately differentiated squamous cell carcinoma of the tongue in 1983 (prior to therapy); described to be tumorigenic in nude mice; listed in the CCLE (ref 18232) with genomic and protein analyses
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at 2-4 x 106 cells/175 cm2; split confluent culture 1:3 to 1:5 every 4-5 days using trypsin/EDTA (for about 10 min)
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 40 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy 49(45-49)<2n>XY/XYY, +7, +9, -18, +20, +mar, add(X)(p22), add(3)(p22-24), i(7p), i(8q), add(9)(p24), del(9)(p2?2) - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list