CAL-62
ACC 448
Cell line:
CAL-62
DSMZ no.:
ACC 448
Species:
human (Homo sapiens)
Cell type:
thyroid anaplastic carcinoma
Origin:
established from the thyroid gland (right lobe) of a 70-year-old woman with thyroid anaplastic carcinoma in 1988; described as being tumorigenic in heterotransplanted nude mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-95% DMEM + 5-10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:10 to 1:15 every 3-5 days using trypsin/EDTA; maximal density at ca. 14 x 106 cells/80 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 28 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human, flat-moded hypertriploid karyotype with 8% polyploidy - 60-76<3n>XX, -X, +3, +3, -4, +6, +7, +9, -11, -14, -18, +19, +20, +20, +2-6mar - del(1)(p34), der(1)t(1;17)(p34;q21), der(3)add(3)(p11)?dup(3)(q21q22)x2-4, del(6)(q15)x2, i(8q), der(?12)t(1;12)(q11-12;q14), add(17)(q21), add(21)(q22) - sideline with del(X)(q24), der(7;8)(q10;q10) - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list