CAL-120

human (Homo sapiens)

breast adenocarcinoma

established from the pleural effusion of a 43-year-old woman with metastatic invasive breast adenocarcinoma in 1991 after radiotherapy; listed in the CCLE (ref 18232) with genome and protein analyses; described as triple-negative; RNA sequence data are available (see Ref 40906 and RNA-Seq)

18232, 40405, 40404, 40906

1

A

heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers; image; image

90% DMEM + 10% h.i. FBS

seed out at ca. 1 x 10⁶ cells/175 cm²; split confluent culture 1:2 every 2-3 days using trypsin/EDTA

at 37 °C with 10% CO₂

ca. 50-60 hours

cell harvest of ca. 6-8 x 10⁶ cells/175 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, RNA hybridization, PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hypertetraploid karyotype with 20% polyploidy 98(88-98)<4n>XXX/XXXX, -1, -2, -2, -4, +5, +5, +6, -9, -9, +10, +11, -12, -12, -13, -13, -14, -15, -16, -16, -20, -21, -22, +16-18mar, + 1-2 dmin, add(X)(p2?2), add(1)(q22), del(3)(q22), add(3)(p1?4), del(4)(p15.2), add(6)(q11)x2, del(7)(q32), add(8)(p12), add(8)(q24), der(8;13)(q10;q10), ins(11;?)(q13;?), add(12)(p1?), add(13)(p11)x1-2, add(14)(p11), der(15;?21)(p1?1;?q12)x3 - sideline with i(1)(p10)add(1)(p3?2), i(1q), i(3p), der(16)t(5;16)(q13;q24)

triple-negative confirmed by absence of ER, PR and HER2 in Western blots

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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