GH3
ACC 469
Cell line:
GH3
DSMZ no.:
ACC 469
Species:
rat (Rattus norvegicus)
Cell type:
pituitary tumor
Origin:
derived in 1965 from a pituitary tumor induced in a female Wistar/Furth rat following repeated transplantation with the MtT/W5 tumor (originally induced in a female F/Fu rat by cranial X-irradiation)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
82.5% Ham's F10 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine
Subculture:
split confluent culture 1:2 to 1:5 every 2-5 days, cells may be detached using trypsin/EDTA or mechanically by agitation; seed out at ca. 4 x 106 cells/80 cm2; maintain at 0.3-0.5 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-90 hours
Harvest:
maximal density of 2 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, and continuously in PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
hypertriploid rat karyotype with 20% poylpoidy - 68-71<3n>
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list