RH-18
ACC 491
Cell line:
RH-18
DSMZ no.:
ACC 491
Species:
human (Homo sapiens)
Cell type:
rhabdomyosarcoma
Origin:
cell line was derived from the tumor material xenografted in immuno-deficient mice; tumor material: primary pediatric embryonal rhabdomyosarcoma exhibiting moderate histological differentiation
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions.
DSMZ Cell Culture Data:
Morphology:
llarge transparent epitheloid cells, partially with long extensions, growing as monolayers; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/175cm2; cells grow rather slowly after thawing; later, split culture 1:3 every 3-6 days (before reaching confluency) using trypsin/EDTA; confluency of the culture might result in reduced growth rate after passaging.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-70 hours
Harvest:
maximum cell harvest of ca. 4 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list