RH-1
ACC 493
Cell line:
RH-1
DSMZ no.:
ACC 493
Species:
human (Homo sapiens)
Cell type:
Ewing sarcoma
Origin:
established from the primary tumor of a young boy patient; originally described as an embryonal rhabdomyosarcoma obtained as xenograft. However, according to gene expression profiling and expression of EWS-FLI1 gene fusion transcripts, the cell line is evidently related to Ewing sarcoma; the cell line is also named EW8(Rh1) or EW-8 in literature
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:4 to 1:20 every 3-10 days using trypsin/EDTA (short exposure only); seed out at ca. 0.5-1.0 x 106 cells/80 cm2; maximal density at ca. 4 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 1-2 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 12% polyploidy - 67(61-67)<3n>XX, -Y, +2, -3, +7, -8, -10, +12, -14, -15, -16, +19, -22, add(X)(p22), del(1)(p3?5p3?6), del(2)(p14)x1-2, del(2)(q23), del(9)(p11p13), ?add(11)(q25)x2, der(11)t(2;11)(q?3?q?3?)t(2;3)(q3?;p21), add(12)(q13)/dup(12)(q13q21), del(13)(?q21), der(14)t(Y;14)(q11.2;p11)x2, der(16)t(16;22)(q22;q22)x2, del(18)(q21), add(19)(q13), del(22)(q13) - sideline with +7, +21, add(2)(q11), add(13)(p11) - absence of t(2;13) confirmed by FISH
Molec. Genetics:
expression of fusion gene EWS-FLI1 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list