Species:
human (Homo sapiens)
Cell type:
histiocytic lymphoma
Origin:
established from the pleural effusion of a 37-year-old man with generalized diffuse histiocytic lymphoma in 1974; cells were described in the literature to express markers and properties of monocytes; patented cell line. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-40 hours
Harvest:
saturation density at about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD54 +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype - 63(58-69)<3n>XXY, -2, -4, -6, +7, -9, -20, -21, +3mar, t(1;12)(q21;p13), der(5)t(1;5)(p22;q35), add(9)(p22), t(10;11)(p14;q23), i(11q), i(12p), add(16)(q22), add(19)(q13) - carries t(10;11) (seen in AML M5) - t(1;5) resembles variant of t(2;5) (histiocytic lymphoma)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
