ALL-SIL
ACC 511
Cell line:
ALL-SIL
DSMZ no.:
ACC 511
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 17-year-old man with T-ALL (T cell acute lymphoblastic leukemia) at relapse; also found in the literature as SIL-ALL; carries NUP214-ABL1 fusion gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
mostly round cells growing singly or in small clusters in suspension; image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; maximal density of ca. 1.5 x 106 cells/ml; it may be of advantange to first culture the cells after thawing in a 24-well-plate with 20% FBS
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
cyCD3 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertetraploid karyotype - 90-95<4n>XX/XXYY, +6, +8, +8, t(1;13)(p32;q32)x2, del(6)(q25)x2, del(9)(?p23p24)x2, t(10;14)(q24;q11.2)x2, add(17)(p11)x2 - sideline with idem, -20, -20 - carries t(10;14) effecting TLX1-TRD (HOX11-TCRD) juxtaposition - matches published karyotype
Molec. Genetics:
expression of TLX1 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list