HCC-1937
ACC 513
Cell line:
HCC-1937
DSMZ no.:
ACC 513
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established in 1995 from a primary tumor of a ductal invasive breast carcinoma of a 24-year-old woman; cells were described as triple-negative but as homozygous for the BRCA1 mutation; listed in the CCLE (ref 18232); a paired EBV+ B-lymphoblastoid cell line (B-LCL HCC-1937BL) is available (DSMZ ACC 891)
; RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
85-90% RPMI 1640 + 10-15% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 2-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of about 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
expression of fusion gene NFIA-EHF was confirmed by RT-PCR; triple-negative confirmed by absence of ER, PR, AR and weak expression of HER2 in Western blots
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list