TC-71
ACC 516
Cell line:
TC-71
DSMZ no.:
ACC 516
Species:
human (Homo sapiens)
Cell type:
Ewing's sarcoma
Origin:
established from the tumor of a 22-year-old man with metastatic Ewing's sarcoma that arose in the humerus; cell line was derived in 1981 from a biopsy of recurrent tumor at the primary site
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
loosely adherent cells growing in monolayers; image
Medium:
90% Iscove's MDM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 every 2-4 days; trypsin/EDTA is not necessary, cells can be detached by tapping the flask
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of about 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 4% polyploidy - 70-80<3n>XX, -Y, +1, +2, -3, +6, +8, -9, +11, -17, +21, +2-11 mar, add(3)(q29), add(6)(q25)x2-3, del(8)(p22), add(8)(p22), del(9(p24), t(11;22)(q24;q12)x2, i(13q)x1-2, add(14)(p11) - sideline with der(2)t(2;3)(p23;p11), add(11)(p15)x2 - carries two copies of t(11;22) effecting EWS-FLI-1 fusion, yielding type 1 (shorter) product - broadly resembles published karyotype
Molec. Genetics:
expression of fusion gene EWS-FLI (type 1) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list