
KCL-22
ACC 519
Cell line:
KCL-22
DSMZ no.:
ACC 519
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the pleural effusion of a 32-year-old woman with Philadelphia chromosome-positive CML in blast crisis in 1981; described in the literature to contain the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene and a p53 mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.5 x 106 cells/ml; split saturated culture 1:5 to 1:10 every 2-3 days; maximal density of ca. 2.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of about 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent (MRA), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD25 -, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 3.3% polyploidy - 51(47-51)<2n>X, -X, +1, +6, +8, +8, +14, +22, del(1)(p22), t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11) - carries Phx2 - matches published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list