CL-34
ACC 520
Cell line:
CL-34
DSMZ no.:
ACC 520
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from a woman with a moderately differentiated primary adenocarcinoma of the right colon (TNM stage 2)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% mixture of DMEM + Ham's F12 at 1:1 + 20% h.i. FBS
Subculture:
seed out at ca. 4 x 106 cells/80 cm2; split semi-confluent culture 1:4 to 1:6 once or twice a week using trypsin/EDTA for 10 min; after thawing the use of collagen-coated flasks for the first week is strongly recommended
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
cell harvest of ca. 15 x 106 cells in a 175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypodiploid karyotype with 16%polyploidy - 45(42-52)<2n>X/XX, -6 - sdl with +3, del(3)(q21) - near-normal "unstable" karyotype with many inconsistent structural and numerical changes
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list