OCI-M1
ACC 529
Cell line:
OCI-M1
DSMZ no.:
ACC 529
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from erythroleukemia blasts (AML M6) of a 62-year-old patient following a 7-year chlorambucil treatment for chronic lymphocytic leukemia (CLL); described in the literature to express many markers of the erythroid lineage as well as several non-erythroid markers
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% Iscove's MDM + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.3 x 106 cells/ml; split saturated culture 1:2 to 1:4 every second day; maximal density of ca. 1 x 106 cells/ml; immediately after thawing viability may be between 70 and 80%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD4(+), CD13(+), CD14-, CD15(+),
CD19-, CD33+, CD34-, CD41(+), CD42-,
CD71+, CD235+; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list