AMO-1
ACC 538
Cell line:
AMO-1
DSMZ no.:
ACC 538
Species:
human (Homo sapiens)
Cell type:
plasmacytoma
Origin:
established from the ascitic fluid of a 64-year-old woman with plasmacytoma (IgAkappa) of the duodenum two months following resection of the tumor mass (additional therapy not specified) in 1984; plasmacytoma is related to multiple myeloma; described to express CD4
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.8 x 106 cells/ml; maintain at ca. 0.5-1.2 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days; minimum density at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70-90 hours
Harvest:
maximum cell density of ca. 1.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, cy IgG -, cy IgM -, cy kappa +, cy lambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list