H-1963
ACC 540
Cell line:
H-1963
DSMZ no.:
ACC 540
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
derived from the lung of a 56-year-old man with small cell lung carcinoma prior to treatment in 1988 (corresponds to NCI-H1963); listed in the CCLE (ref 18232) with genome and protein analyses
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
cells grow in clumps in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split culture 1:2 every 3 days; seed out at ca. 0.5 x 106 cells/ml; cell number is difficult to determine due to the formation of large aggregates
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-4 days
Harvest:
cell harvest of ca. 1-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human bimodal hypodiploid/hypotetraploid aryotype with 3% polyploidy - 44(42-48)<2n>X, -Y, -5, -10, -13, +18, -19, +2mar, add(1)(q32), ins(2;hsr)(p21-23;?), add(3)(p11), del(4)(q24-26), del(10)(p13-14), add(18)(p11)x2, del(20)(q12), der(22)(t1;22)(q12;q13), dmin present in some cells
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list