RCH-ACV
ACC 548
Cell line:
RCH-ACV
DSMZ no.:
ACC 548
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from bone marrow cells taken at relapse of common acute lymphoblastic leukemia (cALL), seven months after diagnosis, from an 8-year-old girl treated with combination chemotherapy
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; split saturated culture 1:2 to 1:10 every 2-6 days; maintain at ca. 0.5-2.5 x 106 cells/ml; easy to culture, tolerates low cell densities
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-50 hours
Harvest:
cell harvest of ca. 4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 -, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 2% polyploidy - 43-50<2n>XX, +8, t(1;19)(q23;p13.3) - sideline with idem, +16, +der(19)t(1;19) - carries t(1;19) effecting TCF3-PBX1 (E2A-PBX) fusion - matches published karyotype
Molec. Genetics:
expression of fusion gene TCF3-PBX1 (E2A-PBX) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list