AML-193
ACC 549
Cell line:
AML-193
DSMZ no.:
ACC 549
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from a 13-year-old girl with acute myeloid leukemia (AML FAB M5) at relapse
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round to polymorph cells growing singly or in small clusters in suspension
Medium:
70% Iscove's MDM + 10% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSMZ ACC 35)
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 49(46-52)<2n>XX, +3, +6, +8, +13, i(17q) - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list