A-498
ACC 55
Cell line:
A-498
DSMZ no.:
ACC 55
Species:
human (Homo sapiens)
Cell type:
kidney carcinoma
Origin:
established from the kidney carcinoma of a 52-year-old man in 1973
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS
Subculture:
seed out at ca. 1.0 x 106 cells/80 cm2; split confluent cultures 1:3 to 1:5 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-70 hours
Harvest:
about 2-3 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 7% polyploidy - 72(68-72)<3n>XX, -X, +2, -3, -6, +7, +7, +9, -11, -14, +17, -18, -20, +21, +3mar, dm/r (31%) - der(1)t(1;1)(p32;q12), der(2)t(2;3)(q37;q13), i(8q) - sideline with der(3)t(3;3)(q27;p14)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list