C6
ACC 550
Cell line:
C6
DSMZ no.:
ACC 550
Species:
rat (Rattus norvegicus)
Cell type:
glioma
Origin:
the glial cell strain C6 was cloned from a rat glioma which was induced by N-nitrosomethylurea
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent fibroblastoid cells forming spindles and growing in monolayers
Medium:
82.5% Ham's F12 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 1-3 x 106 cells/175 cm2 flask; split confluent culture up to 1:20 every 2-3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25-30 hours
Harvest:
maximal density at ca. 2.0 x 107 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat hyperdiploid karyotype with 4% polyploidy - 43(40-44)<2n>
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list