KMS-12-BM

human (Homo sapiens)

multiple myeloma

established from the bone marrow of a 64-year-old woman with multiple myeloma in 1988; sister cell line of KMS-12-PE (DSMZ ACC 606). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

14817, 14486, 18187

1

A

mostly single cells in suspension, 1-2% giant cells; image

80% RPMI 1640 + 20% h.i. FBS

seed out at ca. 0.5 x 10⁶ cells/ml; split saturated culture 1:2 every 2-3 days; maintain at ca. 0.5-1.0 x 10⁶ cells/ml; first week after thawing cultivate in 24-well-plate, initially cells grow slowly

at 37 °C with 5% CO₂

ca. 60 hours

cell harvest of ca. 1.5 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human flat-moded hypertriploid karyotype with 3% polyploidy - 69-79<3n>XX, -X, +1, +1, -4, +5, +6, +8, +9, -10, +13, +14, -15, -16, +17, -18, +19, +20, +21, -22, +4-5mar, der(1)t(1;4)(q11;q32), del(1)(p21)x2, der(1;8)(q10;q10)x2, add(4)(q32)x1-2, der(5;17)(p10;q10)x2, add(6)(q2?5), der(9)add(9)(p24)t(9;11)(q34;q13), der(9)t(9;11)(q34;q13), der(11)t(11;14)(q13;q32)x2-3, idic(?;13)(?;q32-33)x2, der(14)t(11;14)(q13;q32), der(17)t(11;17)(q21.2;q22)t(11;14)(q13;q32)x2, add(19)(q13), add(19)(q13) - related to published karyotype - carries semi-cryptic t(11;14) with IGH-CCND1 rearrangement

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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