Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndrome (MDS, refractory anemia with excess of blasts, RAEB); described to carry internal tandem duplication of FLT3; the FLT3 protein is not expressed; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-14 (ACC 777). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
most cells are round growing in suspension;
image;
image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca 0.4-2.0 x 106 cells/ml; viability may drop below 50% after thawing, but cells recover after 4-5 days (starting in a 12- or 24-well plate is recommended)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cy CD68 +, HLA-DR -
;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MOLM-14
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 51(48-52)<2n>XY, +8, +8, +8, +13, del(8)(p1?p2?), ins(11;9)(q23;p22p23) - resembles published karyotype - sideline with idem, +19 - carries occult insertion effecting KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
