Leibniz Institute DSMZ: Details

Cell line:

MOLM-16

DSMZ no.:

ACC 555

Species:

human (Homo sapiens)

Cell type:

acute myeloid leukemia

Origin:

established in 1999 from the peripheral blood of a 77-year-old woman at relapse of acute myeloid leukemia (AML M0) after failed chemotherapy

Reference(s):

14747

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

large round cells growing singly or in small clumps in suspension; image; image; image; image

Medium:

80% RPMI 1640 + 20% h.i. FBS

Subculture:

seed out at ca. 0.5 x 10⁶ cells/ml; maintain at ca 0.2-0.8 x x10⁶ cells/ml; do not exceed 1 x 10⁶ cells/ml; split saturated culture 1:2 to 1:4 every 2-5 days

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 50-80 hours

Harvest:

cell harvest of ca. 1 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD13 +, CD19 -, CD33 +, CD71 +, CD235a +; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypotetraploid karyotype with hypodiploid sideline - 83-88<4n>XXX, -X, -4, -4, -5, +6, +7, -9, -18, +2mar, add(5)(q11)x2, add(6)(q11-13), der6)t(6;9(q16;p13), t(6;8)(q15;q24.3), der(8)t(6;8)(q15;q24.3), der(9;18)(p10;q10), der(16)del(16)(p13)add(16)(q11), der(16)(del16)(p13)dup(16) (q?11q?24)ins(16;?)(q11;??), der(18)t(9;18)(q13;q21), der(19)add(19)(p13)add(19)(q13)x2 - resembles published karyotype

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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