ROS-50
ACC 557
Cell line:
ROS-50
DSMZ no.:
ACC 557
Species:
human (Homo sapiens)
Cell type:
B cell leukemia
Origin:
established from the peripheral blood of a 69-year-old man with B cell acute lymphoblastic leukemia (B-ALL L3) at relapse; reported to carry the BCL2 rearrangement in the minor cluster region (mcr) in contrast to the major breakpoint region (MBR) of most cell lines
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-85% RPMI 1640 + 15-20% h.i. FBS
Subculture:
seed out at ca. 1-1.5 x 106 cells/ml; cells may be difficult to culture; after thawing cells may need 5-7 days to recover completely and need dense contact for optimal proliferation; in exponential growth split culture 1:2 to 1:3 every 2-3 days; maintain at 0.8 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 1.5-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 12% polyploidy - 45-48<2n>XY, +7, +8, der(7;17)(p10;q10), t(8;14;18)(q24;q32;q21), der(8)t(8;14;18) - carries rearrangements of BCL2, IGH and MYC - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list