Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 65-year-old woman with T cell large granular lymphocyte leukemia (T-CLL or T-LGL) in chronic phase in 2001. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
single or clustered ovoid to round, sometimes irregularly shaped cells, growing in suspension;
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Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS + 5ng/ml IL-2
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximal cell harvest of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 +, sm/cyCD3 -, CD4 +, CD5 -, CD6 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -;
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat moded near-diploid karyotype with 4% polyploidy - 40-47<2n>XX, (1;14)(p36;q12), der(2)t(X;2)(q37;q26), t(2;6)(q32;q24), t(11;17)(p15;q11), t(12;18)(q11;q21) - resembles published karyotype
Molec. Genetics:
expression of fusion gene TBL1XR1::TP63 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
