R1
ACC 56
Cell line:
R1
DSMZ no.:
ACC 56
Species:
fish - Chinook salmon (Oncorhynchus tshawytscha)
Cell type:
liver
Origin:
established from the liver of a Chinook salmon (Oncorhynchus tshawytscha) by spontaneous immortalization. Cell line is used in toxicity assays and waste water control (to reduce fish tests)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Medium 199 (25 mM HEPES) with Earle's salts with L-glutamine + 10% h.i. FBS
Subculture:
cell growth is extremely slow; split confluent culture 1:2 every 7 days using TNE (50 mM Tris, 0.1 M NaCl, 5 mM EDTA, pH 7.5) at +4 °C for 10 min; culture at room temperature (about 20 °C) without CO2 (keep lid of flask closed); cells can be stored temporarily (up to 1 week) at 4 °C; seed out at ca. 0.1 x 106 cells/ml
Incubation:
at room temperature (about 20 °C) without CO2
Doubling time:
about 160 hours
Harvest:
about 5 x 106 cells/175 cm2 - saturation density of ca. 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated by the depositor with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Oncorhynchus tshawytscha
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list